Journal: Biochemistry and Biophysics Reports

Article Title: Characterization and optimization of fluorescein isothiocyanate labeling of humanized h2E2 anti-cocaine mAb

doi: 10.1016/j.bbrep.2023.101520

Figure Lengend Snippet: SDS-PAGE gel analysis of the time course of FITC labeling of the h2E2 mAb shown in . Panels A and B are the fluorescence and the Coomassie staining, respectively, of the intact mAb (2 μg per lane, not reduced) and the heavy chain (HC) and light chain (LC), after reduction of 4 μg per lane of the intact mAb. Panels C and D are the fluorescence and the Coomassie staining, respectively, of the intact mAb (2 μg per lane, not reduced) and the Fab and Fc fragments after proteolysis of mAb with Endo Lys-C (4 μg per lane, not reduced).

Article Snippet: The SDS-PAGE gel protein standards were from SMOBIO (cat. PM1600).

Techniques: SDS Page, Labeling, Fluorescence, Staining

Journal: PLoS ONE

Article Title: Substitution of Glutamate Residue by Lysine in the Dimerization Domain Affects DNA Binding Ability of HapR by Inducing Structural Deformity in the DNA Binding Domain

doi: 10.1371/journal.pone.0076033

Figure Lengend Snippet: (A) A 12-h grown culture of indicated strains were grown with agitation. Protein samples were prepared from equal number of bacterial cells, analyzed on 13.5% SDS polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently transferred to Immobilon-P membrane. FLAG-tagged HapR proteins were detected using monoclonal HRP-conjugated anti-FLAG antibody. Molecular masses were calculated with reference to SDS-PAGE molecular mass standards. (B) Coomassie-brilliant-blue-stained gel is presented as loading control for western blot experiment. Protein marker sizes are shown to the left (in kDa).

Article Snippet: Molecular masses were calculated with reference to SDS-PAGE molecular mass standards (Broad range) from NAX-GEN ALPHA PS ladder from Genetix biotech.

Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Western Blot, Marker